Promotion of differentiation of dendritic cells
NEOPLASMA,56,2,2009
D. Cholujova et al.
Peripheral Blood Mononuclear Cells were isolated from the blood of healthy subjects and cultured in the presence of GM-CSF and IL-4 for 6 days, to prepare immature dendritic cells (iDC). On the 7th day, BioBran was added at various concentrations to the iDCs. The iDCs were incubated for 2 days and their maturation to dendritic cells was observed. The same operation was performed using two kinds of culture medium which have the effect of promoting the development of iDC to mature dendritic cells, designated matDC1, and matDC2, respectively.
As a result, BioBran suppressed the expression of the monocyte marker CD14 and increased the expression of the dendritic cell marker CD83, both in a dose-dependent manner.
This result suggests that BIoBran promotes the differentiation of dendritic cells.
This figure has been partially modified from the original data for optimal visualization on the website.