Appearance	Light yellow powder
Odor	No odor or slight fermented odor
Moisture	8%or less
Arsenic	1ppm or less
Heavy metal	10ppm or less
Aerobic plate count	3000CFU/g or less
Coliform bacteria	Negative

Storage condition: Avoid high temperature and humidity, and ensure containers are tightly sealed.

(1)	Peptidase Activity (synthetic substrate method) The sample solution is warmed at 37°C with the synthetic chromogenic substrate S-2251 (H-D-valyl-L-leucyl-L-lysine-p-nitroanilide dihydrochloride) as a substrate and the absorbance at 405nm is determined. The enzyme activity is defined as 1 unit when 1nmol of p-nitroaniline per minute is released.
(2)	Bacillus subtilis natto-produced Protein (ELISA) ELISA (enzyme-linked immunosorbent assay) uses rabbit-specific antibodies to the Bacillus subtilis natto-produced protein, responsible for the peptidase activity, to measure the amount of antigen reacting with the specific antibodies.

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